LOCUS       40924_23744       11724 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB719 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11724)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2080..2109
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2116..2145
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2161..2190
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2197..2226
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2233..2262
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2278..2307
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2314..2343
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2350..2379
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2395..2424
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2431..2460
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2488..2740
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2774..2790)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2798..2814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2822..2852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2867..2888)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2924..3176)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3214..4509)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61214.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3214..4509)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4561..4740)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5246..5270)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5791..6579
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6828..7416
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7602..7742)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8086..8280)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8349..9419)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9851..10477)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcgggtta attaacggtg aacaaaagct aatctccgag
     2101 gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg actcgacggt
     2161 gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat ctccgaggaa
     2221 gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact cgacggtgaa
     2281 caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac
     2341 ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggactcga cggtgaacaa
     2401 aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg
     2461 aacggtgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
     2521 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
     2581 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
     2641 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
     2701 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattggt tccggaacca
     2761 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2821 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2881 tcacattagg ctgaattagg cgcgcctatt tctattaatt cccgatctag taacatagat
     2941 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     3001 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     3061 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     3121 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg
     3181 aaattcgcct cgagtgactt ctctgattgc agactattta taccaaccag caagaaagtg
     3241 tcgaagtatg aagcagaagg cgcaggctag agcctggaaa acgagaagat ggatgcaaag
     3301 agacttttct gatttaggac caaacaacct caagtaaaca ttgacgagtg tcccatcttt
     3361 tgtgccggtc agtaatccgg tagcaggatc ataccttggg agacgatggc gcggacattt
     3421 cacaatccca gcaagctgag gaagcgataa tagaaggttc agaacttgag gaagaaagaa
     3481 gatgaggagg gtttcactga agtgacccag aataccaaca actgccatag tcattccggc
     3541 gaagactgtg taagtgtctc caacaaaaac tgaagaagga taccaattgt aagcaagcat
     3601 tgccaaggat gtggccatca gcggttgagt aagaaatatc gagaaggcat gagcttggtg
     3661 atactcggga tcaacagatg ctccgatttg cataacgttg tgtatcagga tagcagcggc
     3721 aataacaact gtttgaccga tttcaaggcc attcagacca gcgtggatgt taatagagtt
     3781 tgtacaaaag acagcaagta gccccatata taacttatat atccttccta gattaagcac
     3841 ttccaagcca atataagcaa caagaggttt tggtataaca atagttgtat gaccagcata
     3901 agccatcaaa agtggaaggg tagcaaaaga tggcaggaca agttttactc tccaaggcac
     3961 atcaaggaca tcatctacaa atccaagcaa aatcatgaag caaatagatg ctagtgctgc
     4021 attatactcc acaagccaat tagaatcttc agtgaagtta aaatactgaa atattatcgc
     4081 cacgatcaaa aagacaatac caacaacaat acccaatgac tctggcactt taatatctcc
     4141 ttgaggcgtg cctcttttgt tgatatcaaa accaaacatg ttacgtctca gaacgtatct
     4201 ggcagtaaca ggaatcattt tgagagtgac gaaaaaccca accaagctaa gccccgcgtt
     4261 aattagaatc gatcgcttga gttcattatc aaccttgtag tgaaaacaga gaaggtatag
     4321 atagagcgaa cagagaagag tagagatcac gaagatcact cctaatttcg gcggcgcgag
     4381 acgaaattcc tcaccggaag cagaaaccgt ctttcgggtc atcttctgct ccgatggcgc
     4441 tgagttgggt tccgacgggt ccggtttgtt gggaattgag atcgatgaag ctctcttacg
     4501 agctgccata tcttattgcc ccccgggatc agatctggat tgagagtgaa tatgagactc
     4561 taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt
     4621 tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg
     4681 tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc
     4741 tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact
     4801 cccttaattc tccgctcatg atcttgatcc cctgcgccat cagatccttg gcggcaagaa
     4861 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     4921 ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact
     4981 gcaagctacc tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     5041 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct
     5101 ttagcagccc ttgcgccctg agtgcttgcg gcagcgtgaa gctgatccgt cgagattaat
     5161 agaaataaat tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
     5221 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     5281 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     5341 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     5401 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     5461 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga
     5521 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     5581 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     5641 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     5701 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     5761 ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg
     5821 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     5881 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     5941 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     6001 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     6061 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     6121 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     6181 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     6241 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     6301 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     6361 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     6421 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     6481 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     6541 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg
     6601 ttcaagccga cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat
     6661 aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa
     6721 taagccctac acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt
     6781 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6841 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6901 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6961 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7021 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7081 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7141 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7201 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7261 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7321 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7381 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7441 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7501 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7561 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     7621 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     7681 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     7741 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7801 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7861 catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac
     7921 ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg
     7981 ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg
     8041 gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg
     8101 cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc
     8161 tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca
     8221 atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca
     8281 ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc
     8341 gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg
     8401 atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg
     8461 atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg
     8521 tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg
     8581 tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg
     8641 tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg
     8701 tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc
     8761 tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc
     8821 gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct
     8881 cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct
     8941 cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc
     9001 aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg
     9061 tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa
     9121 acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg
     9181 aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc
     9241 ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg
     9301 cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc
     9361 aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg
     9421 ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc
     9481 ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg
     9541 gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt
     9601 catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc
     9661 ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag
     9721 ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg
     9781 cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct
     9841 cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag
     9901 cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc
     9961 tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg
    10021 caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta
    10081 gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc
    10141 atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg
    10201 acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga
    10261 tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat
    10321 cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa
    10381 catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg
    10441 acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt
    10501 atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac
    10561 acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc
    10621 cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg
    10681 cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat
    10741 gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca
    10801 ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc
    10861 cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag
    10921 cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc
    10981 tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct
    11041 cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac
    11101 tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc
    11161 cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc
    11221 agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca
    11281 gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc
    11341 ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag
    11401 tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc
    11461 atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg
    11521 acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc
    11581 gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag
    11641 cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat
    11701 acaaatggac gaacggataa acct
//
